Skip to content
Pharmaceutics

Established Bioanalytical Methods

The following list represents previously developed and validated drug assays established in the UW Pharmacokinetics Laboratory. Most have been validated according to FDA guidelines for bioanalytical methods. For research projects requiring analysis of these drugs, the timeline may be expedited since method development is already complete. Metabolites and other products are listed below the main compound, when applicable.

Alpha-2 Agonists

  • Clonidine (plasma)
      • Hydroxyclonidine
  • Dexmedetomidine (plasma)

Analgesics

  • Aspirin (plasma)
      • Acetylsalicylic acid
      • Salicylic acid
  • Ibuprofen (plasma)
      • Chiral separation

Anesthetics (General)

  • Propofol (plasma)

Anesthetics (Local)

  • Bupivacaine (plasma)
  • Lidocaine (plasma)

Antibiotics

  • Amoxicillin (plasma)

Anticholinergics

  • Atropine (plasma)

Antidepressants

  • Bupropion (plasma)
      • Hydroxybupropion
  • Desipramine (in vitro)
  • Sertraline (plasma)

Antidiabetics

  • Glyburide (plasma)
      • 4-trans-hydroxycyclohexyl glyburide
      • 3-cis-hydroxycyclohexyl glyburide
      • 4-cis-hydroxycyclohexyl glyburide
      • 3-trans-hydroxycyclohexyl glyburide
  • Metformin (plasma, breast milk, urine)
  • Tolbutamide (urine)
      • Hydroxytolbutamide
      • Carboxytolbutamide

Antineoplastics

  • Busulfan (plasma)
      • Tetrahydrothiophene
  • Clofarabine (plasma)
  • Cyclophosphamide (plasma, in vitro)
      • Hydroxycyclophosphamide (HCY)
      • (S)-2-ethyl-1,5-dimethyl-3,3-diphenyl pyrrolidine (S-EDDP)
      • Dechloroethylcyclophosphamide (DCCY)
      • Aldophosphamide (AldoCY)
      • Carboxyethylphosphoramide mustard (CEPM)
      • 4-glutathionylcyclophosphamide (GSCY)
  • Docetaxel (plasma)
  • Doxorubicine (plasma, tissue)
  • Fludarabine (plasma)
  • Melphalan (plasma)
  • Temozolomide (plasma)

Antivirals

  • Nelfinavir (plasma)
  • Efavirenz’
  • Lamivudine
  • Abacavir
  • Bictegravir

Beta Blockers

  • Carvedilol (plasma)
  • Metoprolol (plasma, breast milk, urine)
      • Chiral separation
      • α-hydroxymetoprolol

Calcium Channel Blockers

  • Felodipine (plasma)

Cardiac Glycosides

  • Digoxin (plasma)

CNS Stimulants

  • Caffeine
      • Plasma: 1,7-dimethylxanthine
      • Urine: methylxanthines and methyluric acids

Corticosteroids

  • Cortisol/Cortisone
      • Cortisol (plasma, urine)
      • Cortisone (plasma, urine)
      • 6β-hydroxycortisol (urine)
      • 6β-hydroxycortisone (urine)
  • Prednisone (urine)
  • Prednisolone (urine)

Hormones

  • Epinephrine (plasma)
  • Estradiol (in vitro)
      • Estradiol-2-glucuronide
      • Estradiol-3-glucuronide

Immunosuppressants

  • Mycophenolic acid (plasma)
      • Mycophenolic acid glucuronide
      • Bound vs. free separation

Muscle Relaxants

  • Chlorzoxazone (plasma)
      • Hydroxychlorzoxazone

NMDA Receptor Antagonists

  • Memantine (plasma)

Opioids

  • Fentanyl (plasma)
  • Methadone (plasma)
  • Morphine (plasma)
      • Morphine-3-glucuronide
      • Morphine-6-glucuronide
  • Oxycodone (plasma)
      • Oxymorphone
      • Noroxycodone
      • Noroxymorphone
  • Remifentanyl (plasma)
  • Hydroxymorphone (plasma)

Proton Pump Inhibitors

  • Omeprazole (plasma)
      • Hydroxyomeprazole

Selective Estrogen Receptor Modulators (SERMs)

  • Tamoxifen (plasma)
      • 4-hydroxytamoxifen
      • Endoxifen
      • n-Desmethyltamoxifen

Statins

  • Pravastatin (plasma)

Supplements

  • Coenzyme Q10
      • Reduced “ubiquinol” and oxidized “ubiquinone” separation
  • Curcuminoids (plasma)
      • Curcumin
      • Desmethylcurcumin
      • Bisdesmethylcurcumin
  • Garlic metabolites (breath)
      • Allyl methylsulfide
      • Diallyl sulfide
      • Diallyl disulfide
      • Diallyl trisulfide
  • Hypericin (plasma)
  • N-acetyl-S-allyl cysteine (urine)
  • S-allyl cysteine (plasma)
  • Nicotinamide riboside (whole blood)
      • Nicotinamide adenosine dinucleotide
      • Nicotinamide mononucleotide

Other

  • Dextromethorphan (in vitro)
  • 4β-Hydroxycholesterol (plasma)
  • Xanthosine monophosphate (lymphocytes)