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Department of Medicinal Chemistry

Mass Spectrometry Center

Book Lab

Located in I-court (I – 093) of the Health Sciences Building on the University of Washington campus (click here for directions), the Mass Spectrometry Center is an open-access self-sustaining instrument laboratory providing a wide variety of mass spectral services to the University and the research community-at-large (including commercial) on a recharge basis.

The Mass Spectrometry Center offers the following services:

  1. Instrument Training – Training services available to center users:
    • Basic UPLC operation and gradient development
    • Compound optimization for quantitative assays
    • Basic mass spectrometry analysis on all center platforms
    • Software training for quantitative and qualitative analysis software
  2. Submitted Sample Analysis – Examples of routine analysis include:
    • High resolution accurate mass measurements of small molecules
    • Lipids, proteins, and small molecules
    • Protein digest peptide mapping (discovery proteomics)
    • Whole protein mass determination
    • LC-MS separations of complex mixtures
    • GC-MS
  3. Assay Development ­– Contracted development of quantitative (MRM) and qualitative assays:
    • Complete quantitative assays including compound optimization and separation methods
    • Extraction protocol development for quantitative assays including recovery calculations
    • Calibration curve development for absolute quantification
  4. MRM Quantitative Proteomics – We offer support on quantitative proteomics including:
    • Development of quantification methods and digests for proteins
    • Quantification in tissues, cells, cell lines, biofluids

About the Center

The Center serves as a resource offering analytical and instructional expertise in contemporary mass spectrometric techniques and instrumentation with the primary aim of providing researchers with “hands-on” training and ready access to “state-of-the-art” instrumentation needed to develop and meet their research interests. Examples of routine analysis are: Low and High Resolution mass analysis, metabolomics, Metid, analyte quantitation, and structural elucidation.

Publication Highlights

Repeated isolation of an antibiotic-dependent and temperature-sensitive mutant of Pseudomonas aeruginosa from a cystic fibrosis patient. Wolter DJ, Scott A, Armbruster CR, Whittington D, Edgar JS, Qin X, Buccat AM, McNamara S, Blackledge M, Waalkes A, Salipante SJ, Ernst RK, Hoffman LR. J Antimicrob Chemother. 2021 Feb 11;76(3):616-625. PubMed link.

Droplet delivery and nebulization system using surface acoustic wave for mass spectrometry. Sun D, Böhringer KF, Sorensen M, Nilsson E, Edgar JS, Goodlett DR. Lab Chip. 2020 Aug 26;20(17):3269-3277. PubMed link.

Structure-Activity Relationships for CYP4B1 Bioactivation of 4-Ipomeanol Congeners: Direct Correlation between Cytotoxicity and Trapped Reactive Intermediates. Kowalski JP, McDonald MG, Whittington D, Guttman M, Scian M, Girhard M, Hanenberg H, Wiek C, Rettie AE. Chem Res Toxicol. 2019 Dec 16;32(12):2488-2498. PubMed link.

Quantitative analysis of chondroitin sulfate disaccharides from human and rodent fixed brain tissue by electrospray ionization-tandem mass spectrometry. Alonge KM, Logsdon AF, Murphree TA, Banks WA, Keene CD, Edgar JS, Whittington D, Schwartz MW, Guttman M. Glycobiology. 2019 Nov 20;29(12):847-860.  PubMed link.

Glycan targeted polymeric antibiotic prodrugs for alveolar macrophage infections. Chen J, Su FY, Das D, Srinivasan S, Son HN, Lee B, Radella F 2nd, Whittington D, Monroe-Jones T, West TE, Convertine AJ, Skerrett SJ, Stayton PS, Ratner DM. Biomaterials. 2019 Mar;195:38-50.  PubMed link.


documentClick here for complete list Mass Spec Publications