The Pharmacokinetics Laboratory is equipped with the following instruments:
An Agilent 6410 triple-quadrupole mass spectrometer coupled to an Agilent 1290 series HPLC is available. This instrument offers the highest sensitivity and specificity of the HPLC-MS systems in the lab. It is used for quantitation of drugs and their metabolites in the concentration range of high fg/ml to low pg/ml range. The mass spectrometer is equipped with electrospray and APCI ionization sources and can handle both negative and positive ions. The 1290 HPLC allows the use of small particle size HPLC columns for high-speed chromatography. Both standard sample vials and 96 well plate sample formats are deployed.
Two single-quadrupole HPLC-MS systems are available: Agilent G1946B and a G1956B. Both instruments are coupled to Agilent 1100 Series HPLCs. These instruments are used for the quantitation of drugs and their metabolites with concentrations in the high pg/ml range. Both mass spectrometers are equipped with electrospray or APCI ionization sources and can handle both negative and positive ions. Both standard sample vials and 96 well plate sample formats are accepted.
A standard Agilent 1100 series HPLC system equipped with single wavelength UV, diode-array UV-Vis, fluorescence, and electrochemical detectors is available for analyses which require the use of HPLC mobile phases that are not amenable to MS detection (e.g., use of ion-pairing reagents). This HPLC accepts standard sample vials.
An Agilent 5973 single-quadrupole mass spectrometer equipped with 6890 GC is available for the analysis of volatile and semi-volatile analytes. The mass spectrometer is equipped with a standard electron impact source for positive ions. Sample introduction includes liquid injection and gas sampling via an Agilent 7694 Headspace sampler or a MARKES sampler which can sample from bags, cartridges, canisters, and on-line gas streams.
AB Sciex 6500 Tandem Quadrupole mass spectrometer. General application: High sensitivity fully automated quantification and screening. Quantitative proteomics, drug metabolism and interaction studies.