Established Drug Assays

The following list represents previously developed and validated drug assays established in the UW Pharmacokinetics Laboratory. Most have been validated according to FDA guidelines for bioanalytical methods. For research projects requiring analysis of these drugs, the timeline may be expedited since method development is already complete. Metabolites and other products are listed below the main compound, when applicable.

  • Amoxicillin (plasma)
  • Aspirin (plasma)
    • Acetylsalicylic acid and salicylic acid
  • Atropine (plasma)
  • Bupivacaine (plasma)
  • Bupropion (plasma)
    • Hydroxybupropion
  • Busulfan (plasma)
    • Tetrahydrothiophene
  • Caffeine (plasma)
    • 1,7-dimethylxanthine
  • Caffeine (urine)
    • methylxanthines
    • methyluric acids
  • Carvedilol (plasma)
  • Clofarabine (plasma)
  • Chlorzoxazone (plasma)
    • Hydroxychlorzoxazone
  • Clonidine (plasma, breast milk, urine)
    • Hydroxyclonidine
  • Coenzyme Q10 (plasma)
    • Reduced “ubiquinol” and oxidized “ubiquinone” separation
  • Cortisol/Cortisone
    • Cortisol (plasma, urine)
    • Cortisone (plasma, urine)
    • 6β-hydroxycortisol (urine)
    • 6β-hydroxycortisone (urine)
  • Curcuminoids (plasma)
    • Curcumin, desmethylcurcumin and bisdesmethylcurcumin
  • Cyclophosphamide (plasma, in vitro)
    • Hydroxycyclophosphamide (HCY), (S)-2-ethyl-1,5-dimethyl-3,3-diphenyl pyrrolidine (S-EDDP), dechloroethylcyclophosphamide (DCCY), aldophosphamide (AldoCY), carboxyethylphosphoramide mustard (CEPM) and 4-glutathionylcyclophosphamide (GSCY)
  • Desipramine (in vitro)
  • Dexmedetomidine (plasma)
  • Dextromethorphan (in vitro)
    • Dextrorphan
  • Digoxin (plasma)
  • Docetaxel (plasma)
  • Doxorubicine (plasma, tissue)
  • Epinephrine (plasma)
  • Estradiol (in vitro)
    • Estradiol-2-glucuronide and estradiol-3-glucuronide
  • Felodipine (plasma)
  • Fentanyl (plasma)
  • Fludarabine (plasma)
  • Garlic metabolites (breath)
    • Allyl methylsulfide, diallyl sulfide, diallyl disulfide and diallyl trisulfide
  • Glyburide (plasma)
    • 4-trans-hydroxycyclohexyl glyburide, 3-cis-hydroxycyclohexyl glyburide, 4-cis-hydroxycyclohexyl glyburide and 3-trans-hydroxycyclohexyl glyburide
  • 4β-hydroxyCholesterol (plasma)
  • Hypericin (plasma)
  • Ibuprofen (plasma)
    • Chiral separation
  • Lidocaine (plasma)
  • Melphalan (plasma)
  • Memantine (plasma)
  • Metformin (plasma, breast milk, urine)
  • Methadone (plasma)
  • Metoprolol (plasma, breast milk, urine)
    • Chiral separation
    • α-hydroxymetoprolol
  • Midazolam (plasma)
    • Hydroxymidazolam
  • Morphine (plasma)
    • Morphine-3-glucuronide and morphine-6-glucuronide
  • Mycophenolic acid (plasma)
    • Mycophenolic acid glucuronide and bound vs. free separation
  • Nelfinavir (plasma)
  • N-acetyl-S-allyl cysteine (urine)
  • Omeprazole (plasma)
    • Hydroxyomeprazole
  • Oxycodone (plasma)
    • oxymorphone, noroxycodone and noroxymorphone
  • Pravastatin (plasma)
  • Prednisone (urine)
  • Prednisolone (urine)
  • Propofol (plasma)
  • Remifentanyl (plasma)
  • Sertraline (plasma)
  • S-allyl cysteine (plasma)
  • Tamoxifen (plasma)
    • 4-hydroxytamoxifen
    • endoxifen
    • n-desmethyltamoxifen
  • Temozolomide (plasma)
  • Tolbutamide (urine)
    • Hydroxytolbutamide and carboxytolbutamide
  • Xanthosine monophosphate (lymphocytes)