Located in I-court (I – 093) of the Health Sciences Building on the University of Washington campus (click here for directions), the Mass Spectrometry Center is an open-access self-sustaining instrument laboratory providing a wide variety of mass spectral services to the University and the research community-at-large (including commercial) on a recharge basis.
The Mass Spectrometry Center offers the following services:
- Instrument Training – Training services available to center users:
- Basic UPLC operation and gradient development
- Compound optimization for quantitative assays
- Basic mass spectrometry analysis on all center platforms
- Software training for quantitative and qualitative analysis software
- Submitted Sample Analysis – Examples of routine analysis include:
- High resolution accurate mass measurements of small molecules
- Lipids, proteins, and small molecules
- MALDI-TOF
- Protein digest peptide mapping (discovery proteomics)
- Whole protein mass determination
- LC-MS separations of complex mixtures
- GC-MS
- Assay Development – Contracted development of quantitative (MRM) and qualitative assays:
- Complete quantitative assays including compound optimization and separation methods
- Extraction protocol development for quantitative assays including recovery calculations
- Calibration curve development for absolute quantification
- MRM Quantitative Proteomics – We offer support on quantitative proteomics including:
- Development of quantification methods and digests for proteins
- Quantification in tissues, cells, cell lines, biofluids
About the Center
The Center serves as a resource offering analytical and instructional expertise by our excellent staff in contemporary mass spectrometric techniques and instrumentation with the primary aim of providing researchers with “hands-on” training and ready access to “state-of-the-art” instrumentation needed to develop and meet their research interests. Examples of routine analysis are: Low and High Resolution mass analysis, metabolomics, Metid, analyte quantitation, and structural elucidation.
Recent Publications
Mixed evidence for stress effects on cortisol-testosterone coupling in Syrian refugee and Jordanian non-refugee adolescents J. Glass D, Godwin J, Koehn J, Dajani R, Hadfield K, Panter-Brick C, Martin M. Psychoneuroendocrinology. 2026; Jan 01;186:107757. doi: 10.1016/j.psyneuen.2026.107757
Breastfeeding on Treprostinil: New Data, Lingering Questions. Leary PJ, Koehn J, Barros LM, Hogl L, Liston NM, Buber J, Whittington D, Rayner S, Rayner SG. Eur Respir J. 2025; Apr 23:2500049. doi: 10.1183/13993003.00049-2025. Online ahead of print.
An assessment of a GMP schistosomiasis vaccine (SchistoShield®). Kim J, Davis J, Lee J, Cho SN, Yang K, Yang J, Bae S, Son J, Kim B, Whittington D, Siddiqui AA, Carter D, Gray SA. Front Trop Dis. 2024;5:1404943. doi: 10.3389/fitd.2024.1404943. Epub 2024 Jul 29.
DNA-m6A calling and integrated long-read epigenetic and genetic analysis with fibertools. Jha A, Bohaczuk SC, Mao Y, Ranchalis J, Mallory BJ, Min AT, Hamm MO, Swanson E, Dubocanin D, Finkbeiner C, Li T, Whittington D, Noble WS, Stergachis AB, Vollger MR. Genome Res. 2024 Nov 20;34(11):1976-1986. doi: 10.1101/gr.279095.124.
P2X7 receptor-dependent increase in endocannabinoid 2-arachidonoyl glycerol production by neuronal cells in culture: Dynamics and mechanism. Singh S, Sarroza D, English A, Whittington D, Dong A, Malamas M, Makriyannis A, van der Stelt M, Li Y, Zweifel L, Bruchas MR, Land BB, Stella N. Br J Pharmacol. 2024 Aug;181(15):2459-2477. doi: 10.1111/bph.16348. Epub 2024 Apr 6.
A pilot study investigating plasma pharmacokinetics and tolerance of oral capecitabine in carcinoma-bearing dogs. Wetzel S, Fidel J, Whittington D, Villarino NF. BMC Vet Res. 2024 Jan 31;20(1):36. doi: 10.1186/s12917-023-03805-y.
Perampanel’s forgiveness factor in a variable medication adherence paradigm in a rat model of chronic epilepsy. Guignet M, Campbell A, Vuong J, Whittington D, White HS. J Transl Med. 2023 Sep 20;21(1):642. doi: 10.1186/s12967-023-04490-z.
DNA-m6A calling and integrated long-read epigenetic and genetic analysis with fibertools. Jha A, Bohaczuk SC, Mao Y, Ranchalis J, Mallory BJ, Min AT, Hamm MO, Swanson E, Dubocanin D, Finkbeiner C, Li T, Whittington D, Noble WS, Stergachis AB, Vollger MR. bioRxiv [Preprint]. 2023 Dec 11:2023.04.20.537673. doi: 10.1101/2023.04.20.537673. Update in: Genome Res. 2024 Nov 20;34(11):1976-1986. doi: 10.1101/gr.279095.124.
Fibertools: fast and accurate DNA-m6A calling using single-molecule long-read sequencing. bioRxiv. 2023 Jul 6:2023.04.20.537673. doi: 10.1101/2023.04.20.537673. Preprint.
The Effects of Pregnancy on Amino Acid Levels and Nitrogen Disposition. Enthoven LF, Shi Y, Fay EE, Moreni S, Mao J, Honeyman EM, Smith CK, Whittington D, Brockerhoff SE, Isoherranen N, Totah RA, Hebert MF. Metabolites. 2023 Feb 7;13(2):242. doi: 10.3390/metabo13020242.