Skip to content
School of Pharmacy

team: Totah Lab

/research/research-labs/

Kailun (Harrison) Hu

 

 

 

  Harrison joins the Totah Lab from the UW Department of Chemistry, where he is co-mentored by Dr. Dan Fu.

Publications

Facilitated Transport of EGFR Inhibitors Plays an Important Role in Their Cellular Uptake. Wong BS, Dunnington EL, Wu R, Kim JI, Hu K, Ro TH, Fu D. Anal Chem. 2024 Jan 30;96(4):1547-1555. doi: 10.1021/acs.analchem.3c04242. Epub 2024 Jan 12. PMID: 38214696
Measuring Drug Response with Single-Cell Growth Rate Quantification. Xu FX, Wu R, Hu K, Fu D. Anal Chem. 2023 Dec 12;95(49):18114-18121. doi: 10.1021/acs.analchem.3c03434. Epub 2023 Nov 28. PMID: 38016067

Jade Yang

 

 

Publications

Liver CYP4A autophagic-lysosomal degradation (ALD): A major role for the autophagic receptor SQSTM1/p62 through an uncommon target interaction site. He L, Kwon D, Trnka MJ, Liu Y, Yang J, Li K, Totah RA, Johnson EF, Burlingame AL, Correia MA. bioRxiv [Preprint]. 2024 Oct 14:2024.10.14.618315. doi: 10.1101/2024.10.14.618315. PMID: 39464120 Preprint.

Investigating the association between CYP2J2 inhibitors and QT prolongation: a literature review. Wiley AM, Yang J, Madhani R, Nath A, Totah RA. Drug Metab Rev. 2024 Mar 20:1-19. doi: 10.1080/03602532.2024.2329928. Online ahead of print. PMID: 38478383 Review.

Serum Protein Exposure Activates a Core Regulatory Program Driving Human Proximal Tubule Injury. Lidberg KA, Muthusamy S, Adil M, Mahadeo A, Yang J, Patel RS, Wang L, Bammler TK, Reichel J, Yeung CK, Himmelfarb J, Kelly EJ, Akilesh S. J Am Soc Nephrol. 2022 May;33(5):949-965. doi: 10.1681/ASN.2021060751. Epub 2022 Feb 23.

Gutsy science: In vitro systems of the human intestine to model oral drug disposition. Arian CM, Imaoka T, Yang J, Kelly EJ, Thummel KE. Pharmacol Ther. 2022 Feb;230:107962. doi: 10.1016/j.pharmthera.2021.107962. Epub 2021 Aug 31.

Kidney Organoid and Microphysiological Kidney Chip Models to Accelerate Drug Development and Reduce Animal Testing. Chen WY, Evangelista EA, Yang J, Kelly EJ, Yeung CK. Front Pharmacol. 2021 Jul 26;12:695920. doi: 10.3389/fphar.2021.695920. eCollection 2021.

Microphysiological system modeling of ochratoxin A-associated nephrotoxicity. Imaoka T, Yang J, Wang L, McDonald MG, Afsharinejad Z, Bammler TK, Van Ness K, Yeung CK, Rettie AE, Himmelfarb J, Kelly EJ. Toxicology. 2020 Nov;444:152582. doi: 10.1016/j.tox.2020.152582. Epub 2020 Sep 6.

 

 

Max Zeigler

Publications

Plasma epoxyeicosatrienoic acids and diabetes-related cardiovascular disease: The cardiovascular health study. Lemaitre RN, Jensen PN, Zeigler M, Fretts AM, Umans JG, Howard BV, Sitlani CM, McKnight B, Gharib SA, King IB, Siscovick DS, Psaty BM, Sotoodehnia N, Totah RA. EBioMedicine. 2022 Sep;83:104189. doi: 10.1016/j.ebiom.2022.104189. Epub 2022 Aug 2.

Reductions in Hydrogen Sulfide and Changes in Mitochondrial Quality Control Proteins Are Evident in the Early Phases of the Corneally Kindled Mouse Model of Epilepsy. Cho C, Zeigler M, Mizuno S, Morrison RS, Totah RA, Barker-Haliski M. Int J Mol Sci. 2022 Jan 27;23(3):1434. doi: 10.3390/ijms23031434.

Plasma epoxyeicosatrienoic acids and dihydroxyeicosatrieonic acids, insulin, glucose and risk of diabetes: The strong heart study. Lemaitre RN, Jensen PN, Zeigler M, Denham J, Fretts AM, Umans JG, Howard BV, Sitlani CM, McKnight B, Gharib SA, King IB, Siscovick DS, Psaty BM, Sotoodehnia N, Totah RA. EBioMedicine. 2021 Apr;66:103279. doi: 10.1016/j.ebiom.2021.103279. Epub 2021 Mar 19.

Changes in erythrocyte membrane epoxyeicosatrienoic, dihydroxyeicosatrienoic, and hydroxyeicosatetraenoic acids during pregnancy. Somani ST, Zeigler M, Fay EE, Leahy M, Bermudez B, Totah RA, Hebert MF. Life Sci. 2021 Jan 1;264:118590. doi: 10.1016/j.lfs.2020.118590. Epub 2020 Oct 15.

A sensitive and improved throughput UPLC-MS/MS quantitation method of total cytochrome P450 mediated arachidonic acid metabolites that can separate regio-isomers and cis/trans-EETs from human plasma. Zeigler M, Whittington D, Sotoodehnia N, Lemaitre RN, Totah RA. Chem Phys Lipids. 2018 Sep 7. pii: S0009-3084(18)30082-3. doi: 10.1016/j.chemphyslip.2018.09.004. [Epub ahead of print]

Hybrid semiconducting polymer nanoparticles as polarization-sensitive fluorescent probes. Zeigler MB, Sun W, Rong Y, Chiu DT. J Am Chem Soc. 2013 Aug 7;135(31):11453-6. doi: 10.1021/ja404719f. Epub 2013 Jul 29.

Single-cell nanosurgery.  Zeigler MB, Chiu DT. Methods Mol Biol. 2013;991:139-48. doi: 10.1007/978-1-62703-336-7_14.

Multicolor fluorescent semiconducting polymer dots with narrow emissions and high brightness. Rong Y, Wu C, Yu J, Zhang X, Ye F, Zeigler M, Gallina ME, Wu IC, Zhang Y, Chan YH, Sun W, Uvdal K, Chiu DT. ACS Nano. 2013 Jan 22;7(1):376-84. doi: 10.1021/nn304376z. Epub 2013 Jan 2.

A versatile method for generating semiconducting polymer dot nanocomposites. Sun W, Hayden S, Jin Y, Rong Y, Yu J, Ye F, Chan YH, Zeigler M, Wu C, Chiu DT. Nanoscale. 2012 Nov 21;4(22):7246-9. doi: 10.1039/c2nr32055j.

Measurements of the acidification kinetics of single SynaptopHluorin vesicles. Budzinski KL, Zeigler M, Fujimoto BS, Bajjalieh SM, Chiu DT. Biophys J. 2011 Oct 5;101(7):1580-9. doi: 10.1016/j.bpj.2011.08.032.

Probing rotational viscosity in synaptic vesicles. Zeigler MB, Allen PB, Chiu DT. Biophys J. 2011 Jun 8;100(11):2846-51. doi: 10.1016/j.bpj.2011.04.042.

Design of highly emissive polymer dot bioconjugates for in vivo tumor targeting. Wu C, Hansen SJ, Hou Q, Yu J, Zeigler M, Jin Y, Burnham DR, McNeill JD, Olson JM, Chiu DT. Angew Chem Int Ed Engl. 2011 Apr 4;50(15):3430-4. doi: 10.1002/anie.201007461. Epub 2011 Mar 4. No abstract available.

Near-infrared fluorescent dye-doped semiconducting polymer dots. Jin Y, Ye F, Zeigler M, Wu C, Chiu DT. ACS Nano. 2011 Feb 22;5(2):1468-75. doi: 10.1021/nn103304m. Epub 2011 Jan 31.

Bioconjugation of ultrabright semiconducting polymer dots for specific cellular targeting. Wu C, Schneider T, Zeigler M, Yu J, Schiro PG, Burnham DR, McNeill JD, Chiu DT. J Am Chem Soc. 2010 Nov 3;132(43):15410-7. doi: 10.1021/ja107196s.

Laser selection significantly affects cell viability following single-cell nanosurgery. Zeigler MB, Chiu DT. Photochem Photobiol. 2009 Sep-Oct;85(5):1218-24. doi: 10.1111/j.1751-1097.2009.00581.x. Epub 2009 Jun 22.

Rheem Totah

Accepting Students to Lab: Yes

Education

  • B.S. (Honors), 1995, Birzeit University, Palestine
  • Masters (Honors), 2000, University of Kansas
  • Ph.D. (Honors), 2002, University of Kansas

Research Interests

  • Prescription drug negative side effects in the heart, skeletal muscle and bone; associated chemical mechanisms for this toxicity
  • Drug-endogenous substrate interactions
  • Pharmacogenetics

Biography

Dr. Totah obtained her bachelor’s degree in chemistry from Birzeit University in Palestine and her master’s and PhD in medicinal chemistry from the University of Kansas. Dr. Totah is currently a professor in the Department of Medicinal Chemistry. Her research is in the area of prescription drug negative side effects especially in the heart, skeletal muscle and bone. She focuses on researching the chemical mechanisms for this toxicity. Her research is important in guiding research towards medicines with fewer side effects and safer use. Research in the Totah lab is broadly centered on drug-endogenous substrate interactions.

Recent Publications

A Rapid and Reliable Absorbance Assay to Identify Drug-Drug Interactions with Thiopurine Drugs. Russell DA, Stafford C, Totah RA. Metabolites. 2024 Dec 19;14(12):715. doi: 10.3390/metabo14120715. PMID: 39728496

Liver CYP4A autophagic-lysosomal degradation (ALD): A major role for the autophagic receptor SQSTM1/p62 through an uncommon target interaction site. He L, Kwon D, Trnka MJ, Liu Y, Yang J, Li K, Totah RA, Johnson EF, Burlingame AL, Correia MA. bioRxiv [Preprint]. 2024 Oct 14:2024.10.14.618315. doi: 10.1101/2024.10.14.618315. PMID: 39464120 Preprint.

A Rapid and Reliable Absorbance Assay to Identify Drug-Drug Interactions with Thiopurine Drugs. Russell DA, Stafford C, Totah RA. Metabolites. 2024 Dec 19;14(12):715.

Editorial: Cytochromes P450, their modulators and metabolites in cardiovascular function and disease. Adebesin AM, Roman RJ, Campbell WB, Seubert JM, Totah RA. Front Pharmacol. 2024 Dec 3;15:1531166.

Liver CYP4A autophagic-lysosomal degradation (ALD): A major role for the autophagic receptor SQSTM1/p62 through an uncommon target interaction site. He L, Kwon D, Trnka MJ, Liu Y, Yang J, Li K, Totah RA, Johnson EF, Burlingame AL, Correia MA. bioRxiv [Preprint]. 2024 Oct 14:2024.10.14.618315.

A Case to Support the Continued Use of Rifampin in Clinical Drug-Drug Interaction Studies. Bercu JP, Ponting DJ, Ripp SL, Dobo KL, Totah RA, Bolleddula J. Clin Pharmacol Ther. 2024 Jul;116(1):34-37.

Investigating the association between CYP2J2 inhibitors and QT prolongation: a literature review. Wiley AM, Yang J, Madhani R, Nath A, Totah RA. Drug Metab Rev. 2024 Mar 20:1-19.

The methyltransferases METTL7A and METTL7B confer resistance to thiol-based histone deacetylase inhibitors. Robey RW, Fitzsimmons CM, Guiblet WM, Frye WJE, González Dalmasy JM, Wang L, Russell DA, Huff LM, Perciaccante AJ, Ali-Rahmani F, Lipsey CC, Wade HM, Mitchell AV, Maligireddy SS, Terrero D, Butcher D, Edmondson EF, Jenkins LM, Nikitina T, Zhurkin VB, Tiwari AK, Piscopio AD, Totah RA, Bates SE, Arda HE, Gottesman MM, Batista PJ. Mol Cancer Ther. 2023 Dec 28.

Cardioprotective mechanisms of cytochrome P450 derived oxylipins from ω-3 and ω-6 PUFAs. Cho C, Aliwarga T, Wiley AM, Totah RA. Adv Pharmacol. 2023;97:201-227.

METTL7A (TMT1A) and METTL7B (TMT1B) are responsible for alkyl S-thiol methyl transferase activity in liver. Russell DA, Chau MK, Shi Y, Levasseur IN, Maldonato BJ, Totah RA. Drug Metab Dispos. 2023 May 3:DMD-AR-2023-001268.

Effects of Pregnancy on Plasma Sphingolipids Using a Metabolomic and Quantitative Analysis Approach. Enthoven LF, Shi Y, Fay E, Kim A, Moreni S, Mao J, Isoherranen N, Totah RA, Hebert MF. Metabolites. 2023 Sep 21;13(9):1026.

CYP2D6 Activity Is Correlated with Changes in Plasma Concentrations of Taurocholic Acid during Pregnancy and Postpartum in CYP2D6 Extensive Metabolizers. Czuba LC, Malhotra K, Enthoven L, Fay EE, Moreni SL, Mao J, Shi Y, Huang W, Totah RA, Isoherranen N, Hebert MF. Drug Metab Dispos. 2023 Nov;51(11):1474-1482.

Cardioprotective mechanisms of cytochrome P450 derived oxylipins from ω-3 and ω-6 PUFAs. Cho C, Aliwarga T, Wiley AM, Totah RA. Adv Pharmacol. 2023;97:201-227.

METTL7A (TMT1A) and METTL7B (TMT1B) Are Responsible for Alkyl S-Thiol Methyl Transferase Activity in Liver. Russell DA, Chau MK, Shi Y, Levasseur IN, Maldonato BJ, Totah RA. Drug Metab Dispos. 2023 Aug;51(8):1024-1034.

Plasma hydrogen sulfide, nitric oxide, and thiocyanate levels are lower during pregnancy compared to postpartum in a cohort of women from the Pacific northwest of the United States. Zeigler MB, Fay EE, Moreni SL, Mao J, Totah RA, Hebert MF. Life Sci. 2023 Jun 1;322:121625.

The Effects of Pregnancy on Amino Acid Levels and Nitrogen Disposition. Enthoven LF, Shi Y, Fay EE, Moreni S, Mao J, Honeyman EM, Smith CK, Whittington D, Brockerhoff SE, Isoherranen N, Totah RA, Hebert MF. Metabolites. 2023 Feb 7;13(2):242.

Cardiac Disease Alters Myocardial Tissue Levels of Epoxyeicosatrienoic Acids and Key Proteins Involved in Their Biosynthesis and Degradation. Aliwarga T, Dinh JC, Heyward S, Prasad B, Gharib SA, Lemaitre RN, Sotoodehnia N, Totah RA. Int J Mol Sci. 2022 Oct 17;23(20):12433.

Selective deuteration of bupropion slows epimerization and reduces metabolism. Shi Y, Dinh J, Pelletier R, Raccor B, Yusuff N, Morgan AJ, Harbeson S, Uttamsingh V, Totah RA. Bioorg Med Chem Lett. 2022 Nov 15;76:129009.

Plasma epoxyeicosatrienoic acids and diabetes-related cardiovascular disease: The cardiovascular health study. Lemaitre RN, Jensen PN, Zeigler M, Fretts AM, Umans JG, Howard BV, Sitlani CM, McKnight B, Gharib SA, King IB, Siscovick DS, Psaty BM, Sotoodehnia N, Totah RA. EBioMedicine. 2022 Sep;83:104189.

Reductions in Hydrogen Sulfide and Changes in Mitochondrial Quality Control Proteins Are Evident in the Early Phases of the Corneally Kindled Mouse Model of Epilepsy. Cho C, Zeigler M, Mizuno S, Morrison RS, Totah RA, Barker-Haliski M. Int J Mol Sci. 2022 Jan 27;23(3):1434.

Future of Biotransformation Science in the Pharmaceutical Industry. Kramlinger VM, Dalvie D, Heck CJS, Kalgutkar AS, O’Neill J, Su D, Teitelbaum AM, Totah RA. Drug Metab Dispos. 2022 Mar;50(3):258-267.

Full PubMed Listing