Rick Harkewicz

Research Scientist

Department of Medicinal Chemistry, Guttman Lab , Medicinal Chemistry Research Staff

Telephone: (206) 543-9402

Email: rharkew1@uw.edu

Publications

Harkewicz R, Du H, Tong Z, Alkuraya H, Bedell M, Sun W, Wang X, Hsu YH, Esteve-Rudd J, Hughes G, Su Z, Zhang M, Lopes VS, Molday RS, Williams DS, Dennis EA, Zhang K. Essential role of ELOVL4 protein in very long chain fatty acid synthesis and retinal function. J Biol Chem. 2012 Mar 30;287(14):11469-80. Epub 2011 Dec 24.

Dumlao DS, Buczynski MW, Norris PC, Harkewicz R, Dennis EA. High-throughput lipidomic analysis of fatty acid derived eicosanoids and N-acylethanolamines. Biochim Biophys Acta. 2011 Nov;1811(11):724-36. doi: 10.1016/j.bbalip.2011.06.005. Epub 2011 Jun 13.

Harkewicz R, Dennis EA. Applications of mass spectrometry to lipids and membranes. Annu Rev Biochem. 2011;80:301-25. doi: 10.1146/annurev-biochem-060409-092612. Review.

Miller YI, Choi SH, Wiesner P, Fang L, Harkewicz R, Hartvigsen K, Boullier A, Gonen A, Diehl CJ, Que X, Montano E, Shaw PX, Tsimikas S, Binder CJ, Witztum JL. Oxidation-specific epitopes are danger-associated molecular patterns recognized by pattern recognition receptors of innate immunity. Circ Res. 2011 Jan 21;108(2):235-48. doi: 10.1161/CIRCRESAHA.110.223875. Review.

Seimon TA, Nadolski MJ, Liao X, Magallon J, Nguyen M, Feric NT, Koschinsky ML, Harkewicz R, Witztum JL, Tsimikas S, Golenbock D, Moore KJ, Tabas I. Atherogenic lipids and lipoproteins trigger CD36-TLR2-dependent apoptosis in macrophages undergoing endoplasmic reticulum stress. Cell Metab. 2010 Nov 3;12(5):467-82. doi: 10.1016/j.cmet.2010.09.010.

Dennis EA, Deems RA, Harkewicz R, Quehenberger O, Brown HA, Milne SB, Myers DS, Glass CK, Hardiman G, Reichart D, Merrill AH Jr, Sullards MC, Wang E, Murphy RC, Raetz CR, Garrett TA, Guan Z, Ryan AC, Russell DW, McDonald JG, Thompson BM, Shaw WA, Sud M, Zhao Y, Gupta S, Maurya MR, Fahy E, Subramaniam S. A mouse macrophage lipidome. J Biol Chem. 2010 Dec 17;285(51):39976-85. doi: 10.1074/jbc.M110.182915. Epub 2010 Oct 5.

Fang L, Harkewicz R, Hartvigsen K, Wiesner P, Choi SH, Almazan F, Pattison J, Deer E, Sayaphupha T, Dennis EA, Witztum JL, Tsimikas S, Miller YI. Oxidized cholesteryl esters and phospholipids in zebrafish larvae fed a high cholesterol diet: macrophage binding and activation. J Biol Chem. 2010 Oct 15;285(42):32343-51. doi: 10.1074/jbc.M110.137257. Epub 2010 Aug 14.

Miller YI, Choi SH, Fang L, Harkewicz R. Toll-like receptor-4 and lipoprotein accumulation in macrophages. Trends Cardiovasc Med. 2009 Oct;19(7):227-32. doi: 10.1016/j.tcm.2010.02.001. Review.

Bedell M, Harkewicz R, Wang X, Zhang K. Focus on molecules: ELOVL4. Exp Eye Res. 2010 Apr;90(4):476-7. doi: 10.1016/j.exer.2009.10.010. Epub 2009 Oct 22. No abstract available.

Choi SH, Harkewicz R, Lee JH, Boullier A, Almazan F, Li AC, Witztum JL, Bae YS, Miller YI. Lipoprotein accumulation in macrophages via toll-like receptor-4-dependent fluid phase uptake. Circ Res. 2009 Jun 19;104(12):1355-63. doi: 10.1161/CIRCRESAHA.108.192880. Epub 2009 May 21.

Harkewicz R, Hartvigsen K, Almazan F, Dennis EA, Witztum JL, Miller YI. Cholesteryl ester hydroperoxides are biologically active components of minimally oxidized low density lipoprotein. J Biol Chem. 2008 Apr 18;283(16):10241-51. doi: 10.1074/jbc.M709006200. Epub 2008 Feb 8.

Deems R, Buczynski MW, Bowers-Gentry R, Harkewicz R, Dennis EA. Detection and quantitation of eicosanoids via high performance liquid chromatography-electrospray ionization-mass spectrometry. Methods Enzymol. 2007;432:59-82.

Harkewicz R, Fahy E, Andreyev A, Dennis EA. Arachidonate-derived dihomoprostaglandin production observed in endotoxin-stimulated macrophage-like cells. J Biol Chem. 2007 Feb 2;282(5):2899-910. Epub 2006 Nov 29. Erratum in: J Biol Chem. 2007 Sep 28;282(39):29068.

Hartvigsen K, Ravandi A, Harkewicz R, Kamido H, Bukhave K, Hølmera G, Kuksis A. 1 -O-alkyl-2-(omega-oxo)acyl-sn-glycerols from shark oil and human milk fat are potential precursors of PAF mimics and GHB. Lipids. 2006 Jul;41(7):679-93.

Stephens D, Barbayianni E, Constantinou-Kokotou V, Peristeraki A, Six DA, Cooper J, Harkewicz R, Deems RA, Dennis EA, Kokotos G. Differential inhibition of group IVA and group VIA phospholipases A2 by 2-oxoamides. J Med Chem. 2006 May 4;49(9):2821-8.

Raetz CR, Garrett TA, Reynolds CM, Shaw WA, Moore JD, Smith DC Jr, Ribeiro AA, Murphy RC, Ulevitch RJ, Fearns C, Reichart D, Glass CK, Benner C, Subramaniam S, Harkewicz R, Bowers-Gentry RC, Buczynski MW, Cooper JA, Deems RA, Dennis EA. Kdo2-Lipid A of Escherichia coli, a defined endotoxin that activates macrophages via TLR-4. J Lipid Res. 2006 May;47(5):1097-111. Epub 2006 Feb 14.

Boullier A, Friedman P, Harkewicz R, Hartvigsen K, Green SR, Almazan F, Dennis EA, Steinberg D, Witztum JL, Quehenberger O. Phosphocholine as a pattern recognition ligand for CD36. J Lipid Res. 2005 May;46(5):969-76. Epub 2005 Feb 1.

Masselon C, Pasa-Tolić L, Lee SW, Li L, Anderson GA, Harkewicz R, Smith RD. Identification of tryptic peptides from large databases using multiplexed tandem mass spectrometry: simulations and experimental results. Proteomics. 2003 Jul;3(7):1279-86.

Pasa-Tolić L, Harkewicz R, Anderson GA, Tolić N, Shen Y, Zhao R, Thrall B, Masselon C, Smith RD. Increased proteome coverage for quantitative peptide abundance measurements based upon high performance separations and DREAMS FTICR mass spectrometry. J Am Soc Mass Spectrom. 2002 Aug;13(8):954-63.

Harkewicz R, Belov ME, Anderson GA, Pasa-Tolić L, Masselon CD, Prior DC, Udseth HR, Smith RD. ESI-FTICR mass spectrometry employing data-dependent external ion selection and accumulation. J Am Soc Mass Spectrom. 2002 Feb;13(2):144-54.

Masselon C, Tolmachev AV, Anderson GA, Harkewicz R, Smith RD. Mass measurement errors caused by ‘local” frequency perturbations in FTICR mass spectrometry. J Am Soc Mass Spectrom. 2002 Jan;13(1):99-106.