| Manufacturer: |
Waters Corporation, Milford, MA, USA |
| *Mass range: |
-
The TOF mass range is 20 to 100,000 m/z in Resolution mode, and 20 to 32,000 m/z in High Resolution mode. The m/z transmission range for a quadrupole in non-resolving mode is 20 to 32,000 m/z for the 8000 m/z quadrupole.
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| *Resolution: |
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| Ionization (±eV): |
- Z-spray Atmospheric Pressure Ionization (API) Source
- Electrospray & Atmospheric Pressure Chemical Ionization
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| Inlet Systems: |
- Waters Acquity UPLC with Autosampler, Direct Infusion Syringe Pump, Flow Injection Analysis (FIA) and HPLC
- HDX – ACQUITY UPLC® M-Class System with HDX Manager. LEAP Technologies dual arm PAL for automated sample preparation
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| Data System (DS): |
- Windows 7
- MassLynx®, DynamX HDX Data Analysis, IDENTITYE, and ProteinLynx Global SERVER™
- MaxEnt®, BioLynx® ProteinLynx® Global
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| Acquisition Date: |
2014 |
| Funding: |
- School of Pharmacy Dean’s Office
- Mass Spectrometry Center Equipment Budget
- Department of Medicinal Chemistry Chair’s Office
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| Predominant Uses: |
- Data dependent LC/MS/MS analysis
- Metabolomic profiling and bio-marker discovery/validation
- Increased specificity and sample definition by the combining of high-efficiency ion mobility separations (IMS) with quadrupole time-of-flight (Q-TOF) mass spectrometry, enabling the differentiation of analytes in complex matrices by size, shape and charge, accurate mass measurement, and fragment analysis Epitope mapping
- Protein-drug binding
- Protein-protein interactions
- Aggregation
- Effects of mutation on protein conformation and dynamics
- Localization of conformational changes as a result of formulations and stability testing
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