Waters Synapt – G2 HDX QTOF

Waters SYNAPT G2-Si HDMS Ion Mobility TOF/LC-MS/IMS/MS

Manufacturer: Waters Corporation, Milford, MA, USA
*Mass range:
  • The TOF mass range is 20 to 100,000 m/z in Resolution mode, and 20 to 32,000 m/z in High Resolution mode. The m/z transmission range for a quadrupole in non-resolving mode is 20 to 32,000 m/z for the 8000 m/z quadrupole.

  • 50,000 FWHM

Ionization (±eV):
  • Z-spray Atmospheric Pressure Ionization (API) Source
  • Electrospray & Atmospheric Pressure Chemical Ionization
Inlet Systems:
  • Waters Acquity UPLC with Autosampler, Direct Infusion Syringe Pump, Flow Injection Analysis (FIA) and HPLC
  • HDX – ACQUITY UPLC® M-Class System with HDX Manager. LEAP Technologies dual arm PAL for automated sample preparation
Data System (DS):
  • Windows 7
  • MassLynx®, DynamX HDX Data Analysis, IDENTITYE, and ProteinLynx Global SERVER™
  • MaxEnt®, BioLynx® ProteinLynx® Global
Acquisition Date: 2014
  • School of Pharmacy Dean’s Office
  • Mass Spectrometry Center Equipment Budget
  • Department of Medicinal Chemistry Chair’s Office
Predominant Uses:
  • Data dependent LC/MS/MS analysis
  • Metabolomic profiling and bio-marker discovery/validation
  • Increased specificity and sample definition by the combining of high-efficiency ion mobility separations (IMS) with quadrupole time-of-flight (Q-TOF) mass spectrometry, enabling the differentiation of analytes in complex matrices by size, shape and charge, accurate mass measurement, and fragment analysis Epitope mapping
  • Protein-drug binding
  • Protein-protein interactions
  • Aggregation
  • Effects of mutation on protein conformation and dynamics
  • Localization of conformational changes as a result of formulations and stability testing