Bruker AutoFlex II

Bruker AutoFlex II Matrix-Assisted LASER Desorption Ionization – Time of Flight Mass Spectrometer (MALDI-TOF)MS

Manufacturer: Bruker Daltonics, Billerica, MA, USA
Mass range:
  • ~ 120 kDa (Linear Mode)
  • ~ 6,000 Da (Reflectron Mode)
Resolution:
  • > 4,000 @ FWHM* (Linear Mode)
  • > 10,000 @ FWHM* (Reflectron Mode)
Ionization (±eV): Matrix Assisted LASER Desorption (MALDI) – 337 nm Nitrogen laser
Inlet Systems:
  • 384 Microtitre Plate/Target
  • LASER: 337nm Nitrogen
Features:
  • Delayed Extraction (DE)
  • Post Source Decay (PSD)
Data System:
  • Windows 7
  • Bruker Compass 1.0, FlexControl
  • Analysis 2.0 and license
  • ClinProTools 2.0
  • GUI Integrated vacuum measurement and control unit
Acquisition Date: 2004
Funding:
  • Department of Biochemistry
  • Department of Chemistry
  • Department of Medicinal Chemistry
  • Department of Periodontics
  • Department of Oral Biology,
  • Department of Medicine (Infectious Diseases)
  • Center for Ecogenetics and Environmental Health (CEEH)
  • School of Pharmacy and its Drug Metabolism/Transport & Pharmacogenomics Research Program (DMTPR)
  • University of Washington Mass Spectrometry Center
Predominant Uses:
  • Characterization and identification of proteins by peptide mass mapping
  • PSD and peptide sequencing.

*FWHM = full width at half height