Agilent 6520

Agilent 6520 Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) LC/MS

Manufacturer Agilent Technologies, Santa Clara, CA, USA
*Mass range: ~ 20-20000 Daltons (TOF)
*Resolution: Up to 40,000 mass resolution, sub-1-ppm MS and 2-ppm MS/MS mass accuracy
Ionization (±eV):
  • Agilent Jet Stream Technology, Orthogonal Spray Ionization Source
  • Electrospray & Atmospheric Pressure Chemical Ionization
  • MS/MS using a quadrupole, a hexapole – collision cell – and a time-of-flight (TOF)
  • Up to 20 MS or 10 MS/MS spectra per second (32 Gbit/sec data acquisition system)
Inlet Systems:
  • Liquid Chromatograph (LC)
  • Agilent Technologies Series 1200 binary solvent delivery system
  • Thermostated auto sampler compartment (vials & 96 well plates)
  • (Agilent Technologies, Palo Alto, CA)
Data System (DS):
  • Microsoft Windows 7
  • MassHunter Data Acquisition and Processing
Acquisition Date: 2009
Funding: ITHS, Dept of Pharmacy, Dept of Pharmaceutics, Dean of Pharmacy
Predominant Uses:
  • Data dependent LC/MS/MS proteomic analysis
  • Metabonomic profiling and biomarker discovery/validation
  • Increased specificity and sample definition by combining accurate mass measurement with quadrupole time-of-flight (Q-TOF) mass spectrometry